login | register | order now | view cart | feedback
Molecular Biology Tools

Products >  Molecular_Biology_Tools >  Restriction_Enzymes >  Restriction_Enzymes_mn >  NdeI


DNA restriction enzymes from Takara Bio are unsurpassed in quality and purity. We’ve been producing them for over 30 years and were the first manufacturers to offer commercially available restriction enzymes in Japan. Each lot of every DNA restriction enzyme undergoes stringent quality control tests. Takara Bio DNA restriction enzymes are supplied with optimized buffers that provide maximum activity during restriction enzyme digestion. Visit our Restriction Enzyme Applications pages for information on how to digest DNA, relative activity in each Universal Buffer, star activity, buffers for double digestion, effects of DNA methylation, how to inactivate enzymes, and more.

  At-A-Glance   Documents   Images & Data


DNA Restriction Enzyme: NdeI

  • Source: Neisseria denitrificans
  • Concentration: 4-12 units/µl
  • Supplied buffer: H
  • Reaction temperature: 37°C
  • Substrate for unit definition: lambda DNA
  • Ligation-recutting test: Ligation efficiency of the cohesive ended DNA fragments generated by this enzyme is low. More efficient ligation can be achieved by using blunt end ligation reaction conditions.
  • Star activity: Cleavage at alternative site(s) occurs in the presence of Mn2+, high concentrations of glycerol, or at high ionic strength.
  • Notice: Do not dilute. This enzyme is unstable at low concentration. The addition of Triton-X 100 to a final concentration of 0.01% in reaction mixture increases the relative activity up to approx. 150%.

TaKaRa follows a stringent QC process when manufacturing their restriction enzymes. Each lot of every enzyme undergoes four quality tests including: overdigestion, Genome DNA analysis, Ligation-Recutting and pKF3 Cloning.

Cat. # Product Contents Size Price Units Select
1161A Nde I 400 Units $62.00
1161B Nde I 2,000 Units $244.00


Recommended Products
Cat. # Product Contents Size Price Units Select
3412A 1 kb DNA Ladder 50 ug $114.00
2660A Alkaline Phosphatase (Shrimp) 300 Units $167.00
6023 DNA Ligation Kit, Mighty Mix 100 Rxns $193.00
RR001A TaKaRa Ex Taq® DNA Polymerase 250 Units $172.00

Takara Bio USA is a Takara Bio Company © 2018 Privacy Policy | Terms & Conditions | Terms of Use