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Products >  Molecular_Biology_Tools >  Restriction_Enzymes >  Restriction_Enzymes_mn >  Mse_I_RspRS_II

RspRS II - isoschizomer of Mse I

RspRS II, an isoschizomer of Mse I, is a restriction enzyme that cleaves within the recognition sequence 5'-TTAA-3'. RspRS II is exceptionally stable and has an optimal reaction temperature of 60°C. It can be used to pretreat (fragment) genomic DNA prior to enrichment of methylated DNA using the EpiXplore Methylated DNA Enrichment Kit from Clontech. RspRS II (Mse I) is one of over a hundred DNA restriction enzymes  offered by Takara Bio. All Takara restriction enzymes are unsurpassed in quality and purity. We’ve been producing them for over 30 years and were the first manufacturers to offer commercially available restriction enzymes in Japan. Each lot of every DNA restriction enzyme undergoes stringent quality control tests. Mse I is supplied with optimized buffer and BSA to  provide maximum activity during restriction enzyme digestion, and is also supplied with 10X Loading Buffer. Visit the Restriction Enzyme Applications pages for information on how to digest DNA, relative activity in each Universal Buffer, star activity, buffers for double digestion, effects of DNA methylation, how to inactivate enzymes, and more.

  At-A-Glance   Documents


DNA Restriction Enzyme: Mse I (RspRS II)

  • Source: Escherichia coli carrying a plasmid encoding the RspRS II gene
  • Concentration: 10 U per µl
  • Supplied buffer: T + BSA
  • Reaction temperature: 60°C
  • Substrate for unit definition: lambda DNA
  • Effect of DNA methylation: Not sensitive
  • Star activity: Cleavage at alternative site(s) occurs in the presence of high concentrations of glycerol
  • Inactivation: Residual activity observed after EtOH precipitation or incubation at 70°C for 15 minutes. No residual activity observed after phenol treatment.

TaKaRa follows a stringent QC process when manufacturing their restriction enzymes. Each lot of every enzyme undergoes four quality tests including: overdigestion, Genome DNA analysis, Ligation-Recutting and pKF3 Cloning.

Cat. # Product Contents Size Price Units Select
1247A RspRS II (Mse I) 500 Units $67.00


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