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Products >  Molecular_Biology_Tools >  Modifying_Enzymes >  Nucleases >  Cryonase_Cold-Active_Nuclease

Cryonase Cold-Active Nuclease

Cryonase Cold-Active Nuclease is a recombinant endonuclease originally derived from a psychrophile, Shewanella sp., and purified from E. coli. This endonuclease enzyme can complete digestion of all types of DNA and RNA (single-stranded, double-stranded, linear or circularized) at cold temperatures and even in samples placed on ice. As a result, Cryonase Cold-Active Nuclease is frequently used during DNA digestion of mixed samples, such as those that include heat-labile proteins.

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  • DNA digestion of all types of DNA
  • RNA digestion of all types of RNA
  • Enzymatic and other heat-labile reactions


Purified from E. coli


  • Protease activity is less than the detection limit.
  • E. coli genomic DNA contamination is less than the detection limit.




Supplied in a buffer [20 mM NaCl, 10 mM Tris-HCl (pH 7.5), 50% glycerol, 2mM MgCl2]


1. Yang, H. et al. (2012) J. Virol. 86(23):13122-13123.

2. Baldermann, S. et al. (2010) J. Exp. Bot. 61(11):2967-2977.

Unit Definition

One unit is defined as the amount of enzyme required to increase 260 nm absorbance by 0.001 per minute at 37°C and pH 7.5 using salmon sperm DNA as the substrate.

Cat. # Product Contents Size Price License Units Select
2670A Cryonase™ Cold-Active Nuclease 10,000 Units $87.00 License Statements
2670B Cryonase™ Cold-Active Nuclease 50,000 Units $346.00 License Statements



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