Tet-On cell lines are host cell lines that greatly simplify the task of setting up a tetracycline-inducible gene expression system that is activated by adding doxycycline (Dox) to the culture medium. Tet-On 3G cell lines, which are our highest performing pre-made cell lines for this system, are located on a separate page.
Tet-Off Advanced and Tet-Off inducible cell lines are also available, which are activated by removing Dox from the culture medium.
These rigorously tested clonal cell lines:
- Stably express one of three generations of the Tet-On transactivator protein including a limited selection of cell lines that express our premium Tet-On 3G transactivator.
- Allow you to induce expression of your gene-of-interest by adding doxycycline (Dox) to the culture of transfected cells
- Can save you up to two months of time in developing a complete Tet-controlled system
Easily create an inducible system by first placing your gene under control of a tetracycline-responsive promoter (e.g. PTRE3G ), transfecting it into one of these G418-resistant cell lines, and selecting for double-stable clones.