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Inducible Systems

Products >  Inducible_Systems >  Tetracycline-Inducible_Expression >  Tet-On_3G

Tet-On 3G Tetracycline-Inducible Expression Systems

The Tet-On 3G Tetracycline-Inducible Gene Expression Systems are the 3rd generation of the most powerful, versatile, and widely cited inducible mammalian expression systems available. The Tet-On 3G system offers a significant improvement over Tet-On and Tet-On Advanced with significantly reduced basal expression and increased sensitivity to doxycycline, a tetracycline analogue.

For more details, download our Tet-On 3G technical note.

Lowest Background Ever

Mutations within the PTRE3G promoter have reduced background expression from this tetracycline inducible promoter by 5–20-fold compared to our previous tightest promoter, PTight.

Highest Sensitivity

Mutations to create the Tet-On 3G transactivator have significantly increased its sensitivity to the inducer doxycycline (Dox).

Highest Fold Induction

Fold induction refers to the difference between the induced and uninduced states. Since Tet-On 3G retains the high maximal expression demonstrated by all Tet-On and Tet-Off systems but has significantly reduced basal expression compared to its predecessors, the fold induction levels are far higher.

Choice of Tet-On 3G Vector Formats

There are a range of available Tet-On 3G vector formats:

  • The Tet-On 3G Inducible Expression System (EF1α Version), our EF-1 alpha promoter versions of the Tet-On 3G system, provide for consistent long-term expression of the Tet-On 3G transactivator, even in cell types known for their tendency to silence a CMV promoter over time, such as hematopoietic cells and stem cells.
  • Bidirectional promoter versions of the system allow to induce expression of two genes from the same promoter, either two transgenes of your choosing or a single transgene and a fluorescent protein. If your cells turn red or green after adding Dox, this confirms thatyour gene has been turned on and that you have selected ahigh-performing inducible clone.
  • Using IRES technology, we’ve married very tight gene expression control to a bright red fluorescent protein (mCherry) in the Tet-On 3G Inducible Expression System (with mCherry) and a bright green fluorescent protein (ZsGreen1) in the Tet-On 3G Inducible Expression System (with ZsGreen1). 
  • Retroviral and lentiviral formats include the PTRE3GV promoter, which is optimized for use in retroviral and lentiviral vectors. They are supplied with complete viral packaging systems.

Use Tetracycline-Approved Serum for Optimal Results

With the greatly increased sensitivity of the Tet-On 3G System, it is more important than ever that the serum you use for your studies is guaranteed to be tetracycline-free. Only Clontech performs actual inducibility tests on a sensitive Tet inducible cell line in order to provide an absolute guarantee that your serum will not affect basal expression in your Tet-On 3G experiments. Each Tet-On 3G System is supplied with 50 ml of our premium Tet-Approved FBS.

  At-A-Glance   Documents   Images & Data   Resources



  • Inducible expression in mammalian cells
  • Inducible expression in transgenic mice
  • Inducible expression in stem cells and hematopoietic cells (EF-1 alpha promoter version)
  • Co-inducible expression of two genes of interest (bicistronic version)
  • Co-express a fluorescent protein (mCherry and ZsGreen1 versions)

Additional Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.


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