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Genome Editing

Products >  Genome_Editing >  CRISPR_Cas9 >  Resources >  CRISPR_screening_webinar


Lentiviral CRISPR/Cas9 knockout screening made simple

In this webinar, Thomas Quinn, M.S. (Takara Bio USA, Inc.) discusses the design and execution of genome-wide CRISPR/Cas9-based knockout screens and their associated challenges. Learn about:

  • The importance of maintaining balanced sgRNA representation during plasmid library amplification, virus production, and transduction of target cells
  • The design and execution of CRISPR/Cas9-based knockout screens and analysis of screened cell populations using NGS
  • Features and benefits of the Guide-it CRISPR Genome-Wide sgRNA Library System and NGS analysis kit, a complete solution developed with the aim of making lentiviral CRISPR/Cas9 screening accessible to all
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