tdTomato is an exceptionally bright red fluorescent protein—6 times brighter than EGFP. tdTomato’s emission wavelength (581 nm) and brightness make it ideal for live animal imaging studies. The tdTomato fluorescent protein is equally photostable to mCherry (1).
Sequence mutations were introduced to mRFP by the Tsien lab (through directed evolution) in order to produce dTomato. This very bright red fluorescent protein was made even brighter by creating a tandem dimer version, the tdTomato fluorescent protein. Because tdTomato forms an intramolecular dimer, it behaves like a monomer with a very bright red fluorescent signal (1).
tdTomato fluorescent protein can be detected with the DsRed Monoclonal and Polyclonal Antibodies. Alternatively, use the mCherry Monoclonal Antibody to differentiate mCherry (which it detects) from DsRed-Express and/or tdTomato (which it does not). Visit the anti-tdTomato Antibody page.
tdTomato sequence files can be found in the Documents tab below.