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Products >  Fluorescent_Proteins_and_Reporters >  Fluorescent_Proteins_by_Name >  AcGFP1_Fluorescent_Protein

AcGFP1 Fluorescent Protein

AcGFP1 (Aequorea coerulescens GFP) is a monomeric green fluorescent protein with spectral properties similar to those of EGFP. The AcGFP1 fluorescent protein is very stable, allowing you to perform studies over an extended period of time. AcGFP1 can be used to visualize and measure gene expression in a variety of organisms from bacteria to higher plants and animals.

A Better Alternative to EGFP

The AcGFP1 and EGFP sequences have 94% homology at the amino acid level. However, AcGFP1 is a superior alternative for fusion applications because it is a monomer. In contrast, the Aequorea victoria GFPs, AvGFP and EGFP, have been reported to form dimers (1–2).

The monomeric nature of the AcGFP1 fluorescent protein has been confirmed by FPLC gel filtration chromatography, sucrose density gradient ultracentrifugation, and pseudonative gel electrophoresis (please see the Images & Data tab for details).

Anti-AcGFP1 Antibody

We offer four antibodies that detect AcGPF1. Visit the anti-AcGFP1 Antibody page.
  At-A-Glance   Documents   Images & Data   Resources

Features

  • Monomeric green fluorescent protein
  • Similar spectral properties to EGFP
    AcGFP1 excitation and emission maxima: 475 and 505 nm, respectively
    EGFP excitation and emission maxima: 484 and 510 nm, respectively

Applications

References

  1. Jain, R. K. et al. (2001) Biochem. J. 360(Pt. 3):645–649. Oligomerization of green fluorescent protein in the secretory pathway of endocrine cells.
  2. Chen, Y. et al. (2002) Biophys. J. 82(1):133–144. Molecular brightness characterization of EGFP in vivo by fluorescence fluctuation spectroscopy.

Additional Product Information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
 
 
Products
Cat. # Product Contents Size Price License Units Select
632154 pLVX-AcGFP1-N1 Vector 10 ug $676.00 License Statements *
632155 pLVX-AcGFP1-C1 Vector 10 ug $676.00 License Statements *
632468 pAcGFP1 Vector 20 ug $574.00 License Statements *
632469 pAcGFP1-N1 Vector 20 ug $574.00 License Statements *
632470 pAcGFP1-C1 Vector 20 ug $574.00 License Statements *
632426 AcGFP1 Vector Set Each $1,102.00 License Statements *
632431 pAcGFP1-Nuc Vector 20 ug $574.00 License Statements *
632432 pAcGFP1-Mito Vector 20 ug $574.00 License Statements *
632435 pIRES2-AcGFP1 Vector 20 ug $574.00 License Statements *
632482 pAcGFP1-C3 Vector 20 ug $574.00 License Statements *
632483 pAcGFP1-N2 Vector 20 ug $574.00 License Statements *
632484 pAcGFP1-N3 Vector 20 ug $574.00 License Statements *
632485 pAcGFP1-N Vector Set Each $1,002.00 License Statements *
632488 pAcGFP1-Tubulin Vector 20 ug $574.00 License Statements *
632489 pAcGFP1-Hyg-N1 Vector 20 ug $574.00 License Statements *
632490 pAcGFP1-Endo Vector 20 ug $574.00 License Statements *
632491 pAcGFP1-Mem Vector 20 ug $574.00 License Statements *
632492 pAcGFP1-Hyg-C1 Vector 20 ug $574.00 License Statements *
632497 pAcGFP1-1 Vector 20 ug $574.00 License Statements *
632500 pAcGFP1-C In-Fusion® Ready Vector 1 ug $396.00 License Statements *
632501 pAcGFP1-N In-Fusion® Ready Vector 1 ug $396.00 License Statements *
632453 pAcGFP1-Actin Vector 20 ug $574.00 License Statements *
632464 pAcGFP1-Golgi Vector 20 ug $574.00 License Statements *
632505 pRetroQ-AcGFP1-N1 Vector 20 ug $568.00 License Statements *
632506 pRetroQ-AcGFP1-C1 Vector 20 ug $568.00 License Statements *
632509 pAcGFP1-Mem Hyg Vector 20 ug $574.00 License Statements *
632510 pAcGFP1-F Hyg Vector 20 ug $574.00 License Statements *
632511 pAcGFP1-F Vector 20 ug $574.00 License Statements *
632515 pIRES2-AcGFP1-Nuc Vector 20 ug $574.00 License Statements *
632481 pAcGFP1-C2 Vector 20 ug $574.00 License Statements *
631971 pEF1alpha-IRES-AcGFP1 Vector 10 ug $541.00 License Statements *
631973 pEF1alpha-AcGFP1-N1 Vector 10 ug $541.00 License Statements *
631974 pEF1alpha-AcGFP1-C1 Vector 10 ug $541.00 License Statements *
631983 pLVX-EF1alpha-AcGFP1-N1 Vector 10 ug $676.00 License Statements *
631984 pLVX-EF1alpha-AcGFP1-C1 Vector 10 ug $676.00 License Statements *
 
* You must log in if you would like to purchase these products online, since the purchase of these products may be restricted depending on your account type. Researchers at not-for-profit accounts receive a limited use license with their purchase of the product. Researchers at for-profit accounts must obtain a license prior to purchase. For details please contact licensing@clontech.com.

 

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