- Cloning and expression of target genes
- DNA sequencing using M13 primers
- Large DNA insert sequencing using Takara's Deletion Kit for Kilo-Sequencing
- >70% double-stranded, covalently closed circular form I (RF I) DNA.
- Multiple cloning site sequence verified by dideoxy DNA sequencing.
- Restriction site verified by restriction enzyme cleavage.
10 mM Tris-HCl (pH 8.0), 1 mM EDTA
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