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Products >  Cloning_and_Competent_Cells >  Cloning_Vectors >  Ecoli-Aspergillus_Shuttle_Vectors

Pyrithiamine vectors

The pyrithiamine or pPTR vectors are E. coli-Aspergillus shuttle vectors and contain an ampicillin resistance gene (AmpR) as the selection marker for E. coli and a pyrithiamine-resistance gene (ptrA) as the selection marker for Aspergillus. Pyrithiamine is a pyridine analog and inhibitor of thiamine. Because the pPTR vector multiple cloning site (MCS) is located within the lacZ region, the presence or absence of target DNA insert can easily be verified using blue/white screening via bacterial plates containing IPTG and X-Gal.

  At-A-Glance   Documents   Images & Data

Features

  • pPTR I DNA (4,782 bp) – Chromosomal integration vector for Aspergillus sp.
  • pPTR II DNA (10,031 bp) – Autonomously replicating vector for Aspergillus sp.
  • These vectors are supplied in 10 mM Tris-HCl (pH 8.0), 1 mM EDTA

Applications

  • Selection of Aspergillus sp. transformants using pyrithiamine


Notes

Do not use reagents containing thiamine during transformation, as traces of thiamine will decrease the bacterial selection efficiency of pyrithiamine media.

Manufactured by Hakutsuru Sake Brewing Co., Ltd.

Unique restriction sites

  • pPTR I DNA: HindIII, PstI, SmaI, and KpnI
  • pPTR II DNA: HindIII, SmaI, and KpnI


Product citations

Kubodera, T., Yamashita, N. & Nishimura, A. Pyrithiamine resistance gene (ptrA) of Aspergillus oryzae: cloning, characterization and application as a dominant selectable marker for transformation. Biosci. Biotechnol. Biochem. 64, 1416–21 (2000).

Additional product information

Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.



 
 
Products
Cat. # Product Contents Size Price Units Select
3621 pPTR I DNA 20 ug $300.00
3622 pPTR II DNA 20 ug $300.00
 

 

 
 

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