Chromatin immunoprecipitation (ChIP) is used to determine the location of DNA binding sites for a particular protein of interest, in order to understand the protein-DNA interactions that occur inside the nucleus of living cells or tissues. ChIP is particularly relevant for epigenetics studies, because DNA-binding proteins play an important role in the regulation of gene expression in healthy cells and organisms and in medically relevant areas such as cancer and immunology.
The EpiXplore Chromatin Immunoprecipitation ChIP kits use magnetic beads and an optimized protocol to make your assay easier— in fact, it can be completed in less than 6 hr. These ChIP kits also include specially formulated buffers which reduce nonspecific DNA pull-down and enhance signal. A DNA purifying resin enables rapid elution of DNA and direct PCR analysis.
- Use the EpiXplore Chromatin Immunoprecipitation Kit: Protein G to identify protein-DNA interactions via protein G, an immunoglobulin-binding protein.
- Use the EpiXplore Chromatin Immunoprecipitation (ChIP) Assay Kit: Anti-mouse IgG to identify protein-DNA interactions via anti-mouse beads. This kit has significantly higher affinity for mouse antibodies than protein G, leading to high efficiency pull-down assays when using mouse primary antibodies.
Highly Specific Chromatin Immunoprecipitation
The EpiXplore ChIP Assay Kit yields very specific results. It is possible to perform a successful chromatin immunoprecipitation assay from as few as 1.5 x 105 cells with the EpiXplore ChIP Assay Kit (see Data & Images tab).